Measurements of Plasma Elastase ₁-Proteinase Inhibitor Complexes in Patients Receiving Cancer Chemotherapy With Granulocyte Colony-Stimulating Factor

¹ From the First Department of Internal Medicine, Kagawa Medical School, Kagawa, Japan

We monitored the plasma elastase ₁-proteinase inhibitor complex levels in 21 patients with primary lung cancer who received combination chemotherapy with or without recombinant human granulocyte colony-stimulating factor (rhG-CSF), and 15 normal nonsmokers as controls. Of the 21 patients, 14 received combination chemotherapy without rhG-CSF (among them, 6 developed pneumonia) and 7 received combination chemotherapy with rhG-CSF (among them, 1 developed pneumonia). We measured peripheral WBC counts, C-reactive protein (CRP) levels, plasma elastase ₁-proteinase inhibitor complex (complex) levels, and complex/WBC values during cancer chemotherapy. In patients who received cancer chemotherapy without rhG-CSF and had no complications (n=8), WBC values decreased after chemotherapy, and then gradually increased. Complex levels also decreased slightly after chemotherapy and gradually recovered. The value obtained from dividing the complex concentration by WBC count (complex/WBC value) remained stable during cancer chemotherapy. In patients who received cancer chemotherapy with rhG-CSF and had no complications (n=6), WBC values decreased after chemotherapy, and then rapidly increased to abnormally high values. Complex levels also decreased slightly after chemotherapy and rapidly increased to abnormally high values together with the WBC counts. The complex/WBC values remained stable during cancer chemotherapy. In patients who developed pneumonia during cancer chemotherapy with or without rhG-CSF (n=7), their complex levels, complex/WBC values, and CRP levels were elevated at the onset of pneumonia. The maximum complex levels (the highest levels during chemotherapy) were significantly higher in patients who received cancer chemotherapy with rhG-CSF and did not develop pneumonia (583.1±114.5 ng/mL) and in patients who developed pneumonia during cancer chemotherapy (516.7±113.2 ng/mL), compared with normal nonsmokers (130.2±5.5, p<0.01) and patients who received cancer chemotherapy without rhG-CSF and did not develop complications (211.5±23.3, p<0.01). The maximum complex/WBC values were not increased in patients who received cancer chemotherapy with rhG-CSF (0.08±0.01) and patients who received cancer chemotherapy without rhG-CSF (0.092±0.01, p<0.01). The maximum complex/WBC values were significantly higher in patients with pneumonia (0.56±0.12) compared with normal nonsmokers (0.026±0.002, p<0.01) and patients without complications. These findings suggest that although rhG-CSF increases total plasma elastase burden, increased release of neutrophil elastase from individual neutrophils does not take place in vivo in the absence of pneumonia.

Key Words: cancer chemotherapy • complex/WBC values • granulocyte colony-stimulating factor • plasma elastase ₁-proteinase inhibitor complexes

Submitted on March 4, 1994
Accepted on September 19, 2007