| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Guest Access | Sign In via User Name/Password
|
|
|
|||||||
Guest Access | Sign In via User Name/Password |
|||||||||
1 From the Department of Anatomy, Laboratory of Molecular Biology, University of Helsinki, Finland; and the Lung Clinic, University of Tartu, Estonia
2 From the Department of Anatomy, Laboratory of Molecular Biology, University of Helsinki, Finland
3 From the Department of Periodontology, University of Helsinki, Finland
Collagenases in bronchoalveolar lavage fluid (BALF) of patients with bronchiectasis and healthy subjects were characterized using specific functional and immunologic assays. The BAL fluid contained interstitial collagenase and collagenolytic proteinases of bacterial origin. Collagenase activities, obtained after organomercurial activation, correlated with the severity of bronchiectasis. In severe cases, collagenase activities were 3.5x10
7 IU/L/48 h or 4.8x106 IU/g/48 h (p<0.01), in moderate ones 1.74x107 IU/L/48 h or 3.35x106 IU/g/48 h (p<0.05), and in mild cases 0.32x107 IU/L/48 h or 0.7x106 IU/g/48 h (p<0.05). The corresponding activities in healthy control subjects were 0.08x107 IU/L/48 h or 0.13x106 IU/g/48 h. The cellular origin of interstitial collagenase was assessed with doxycycline inhibition test utilizing the differential sensitivity of fibroblast-type collagenase/MMP-1 (IC50=280 µM) and neutrophil-type collagenase/MMP-8 (IC50=26 µM) to the anticollagenolytic, nonantimicrobial doxycycline action. Interstitial collagenase, contained in BALF, was totally inhibited by 100 µM of doxycycline. It can therefore be concluded that most of mammalian collagenase presented in inflamed fluid of bronchiectasis originated from neutrophils. The molecular forms of neutrophil-type collagenase/MMP-8 were confirmed and analyzed by Western-blot, which showed evidence of the proteolytic conversion of the latent 85-kD MMP-8 proenzyme species into active 65-kD molecular weight species. These findings strongly suggest involvement of proteolytic activation pathway of proMMP-8, especially in severe and moderate forms of bronchiectasis. Furthermore, collagenolytic proteases of bacterial origins may also participate in tissue destruction of the lung.
Key Words: BAL • bronchiectasis • collagenolytic proteinases • MMP-8
Submitted on May 10, 1994
Accepted on October 18, 2007
This article has been cited by other articles:
|
|
 |
|
  K. S. Lee, K. H. Min, S. R. Kim, S. J. Park, H. S. Park, G. Y. Jin, and Y. C. Lee Vascular Endothelial Growth Factor Modulates Matrix Metalloproteinase-9 Expression in Asthma Am. J. Respir. Crit. Care Med., July 15, 2006; 174(2): 161 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Rosen Chronic Cough Due to Bronchiectasis: ACCP Evidence-Based Clinical Practice Guidelines Chest, January 1, 2006; 129(1_suppl): 122S - 131S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Gueders, M. Balbin, N. Rocks, J.-M. Foidart, P. Gosset, R. Louis, S. Shapiro, C. Lopez-Otin, A. Noel, and D. D. Cataldo Matrix Metalloproteinase-8 Deficiency Promotes Granulocytic Allergen-Induced Airway Inflammation J. Immunol., August 15, 2005; 175(4): 2589 - 2597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Holopainen, J. A. O. Moilanen, T. Sorsa, M. Kivela-Rajamaki, T. Tervahartiala, M. H. Vesaluoma, and T. M. T. Tervo Activation of Matrix Metalloproteinase-8 by Membrane Type 1-MMP and Their Expression in Human Tears after Photorefractive Keratectomy Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2550 - 2556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.G. Gibson, P.A.B. Wark, J.L. Simpson, C. Meldrum, S. Meldrum, N. Saltos, and M. Boyle Induced sputum IL-8 gene expression, neutrophil influx and MMP-9 in allergic bronchopulmonary aspergillosis Eur. Respir. J., April 1, 2003; 21(4): 582 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Hartog, J. A. Wermelt, C. O. Sommerfeld, W. Eichler, K. Dalhoff, and J. Braun Pulmonary Matrix Metalloproteinase Excess in Hospital-acquired Pneumonia Am. J. Respir. Crit. Care Med., February 15, 2003; 167(4): 593 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F Ratjen, C-M Hartog, K Paul, J Wermelt, and J Braun Matrix metalloproteases in BAL fluid of patients with cystic fibrosis and their modulation by treatment with dornase alpha Thorax, November 1, 2002; 57(11): 930 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A B Chang, N C Boyce, I B Masters, P J Torzillo, and J P Masel Bronchoscopic findings in children with non-cystic fibrosis chronic suppurative lung disease Thorax, November 1, 2002; 57(11): 935 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zheng, W.K. Lam, G.L. Tipoe, I.H. Shum, C. Yan, R. Leung, J. Sun, G.C. Ooi, and K.W. Tsang Overexpression of matrix metalloproteinase-8 and -9 in bronchiectatic airways in vivo Eur. Respir. J., July 1, 2002; 20(1): 170 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Loukides, D. Bouros, G. Papatheodorou, S. Lachanis, P. Panagou, and N. M. Siafakas Exhaled H2O2 in Steady-State Bronchiectasis : Relationship With Cellular Composition in Induced Sputum, Spirometry, and Extent and Severity of Disease Chest, January 1, 2002; 121(1): 81 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Cederqvist, T. Sorsa, T. Tervahartiala, P. Maisi, K. Reunanen, P. Lassus, and S. Andersson Matrix Metalloproteinases-2, -8, and -9 and TIMP-2 in Tracheal Aspirates From Preterm Infants With Respiratory Distress Pediatrics, September 1, 2001; 108(3): 686 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D G Sweet, K J McMahon, A E Curley, C M O'Connor, and H L Halliday Type I collagenases in bronchoalveolar lavage fluid from preterm babies at risk of developing chronic lung disease Arch. Dis. Child. Fetal Neonatal Ed., May 1, 2001; 84(3): 168F - 171. [Abstract] [Full Text]
|
|
|
|
|
|
D. K. Y. SHUM, S. C. H. CHAN, and M. S. M. IP Neutrophil-Mediated Degradation of Lung Proteoglycans . Stimulation by Tumor Necrosis Factor-alpha in Sputum of Patients with Bronchiectasis Am. J. Respir. Crit. Care Med., November 1, 2000; 162(5): 1925 - 1931. [Abstract] [Full Text]
|
|
|
|
|
|
G. Mautino, C. Henriquet, D. Jaffuel, J. Bousquet, and F. Capony Tissue Inhibitor of Metalloproteinase-1 Levels in Bronchoalveolar Lavage Fluid from Asthmatic Subjects Am. J. Respir. Crit. Care Med., July 1, 1999; 160(1): 324 - 330. [Abstract] [Full Text]
|
|
|
|
|
|
S. LOUKIDES, I. HORVATH, T. WODEHOUSE, P. J. COLE, and P. J. BARNES Elevated Levels of Expired Breath Hydrogen Peroxide in Bronchiectasis Am. J. Respir. Crit. Care Med., September 1, 1998; 158(3): 991 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Hanemaaijer, T. Sorsa, Y. T. Konttinen, Y. Ding, M. Sutinen, H. Visser, V. W. M. van Hinsbergh, T. Helaakoski, T. Kainulainen, H. Ronka, et al. Matrix Metalloproteinase-8 Is Expressed in Rheumatoid Synovial Fibroblasts and Endothelial Cells. REGULATION BY TUMOR NECROSIS FACTOR-alpha AND DOXYCYCLINE J. Biol. Chem., December 12, 1997; 272(50): 31504 - 31509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O Eickelberg, C. Sommerfeld, C Wyser, M Tamm, F Reichenberger, P. Bardin, M Soler, M Roth, and A. Perruchoud MMP and TIMP expression pattern in pleural effusions of different origins Am. J. Respir. Crit. Care Med., December 1, 1997; 156(6): 1987 - 1992. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
