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1 From the Department of Anesthesiology, State University of New York, SUNY School of Medicine, Buffalo
2 From the Department of Pathology, University of Michigan, Ann Arbor, Mich.
Objectives: To examine the effects of an increase in ambient oxygen (O2) concentrations on the extent of inflammatory pulmonary damage following acid aspiration.
Design: Prospective, controlled laboratory study.
Settings: University-affiliated animal research facility.
Subjects: Male, Long Evans rats weighing 250 to 300 g.
Intervention: Rats were injured by instillation of 1.2 mL/kg normal saline solution/HCl, pH=1.25 (acid), into the lungs via a tracheotomy. Animals were allowed to awaken and were exposed to 21%, 50%, or 98% O2 for 0 to 5 h (n/group
10). In a separate set of experiments, injured rats exposed to 98% O2 were treated with different doses of deferoxamine, just prior to injury. Uninjured rats and rats injured with normal saline solution, pH=5.3, were used as the control group.
Measurements: Injury was determined by assessing lung function (lung compliance and arterial blood gases) and alveolar-capillary wall integrity (wet/dry weight, lung albumin permeability index [PI], and intrapulmonary hemorrhage [HI]).
Results: Intrapulmonary instillation of acid increased PI, HI, and decreased static lung compliance compared to uninjured control animals. Increased ambient oxygen following acid aspiration decreased lung compliance, 1.06±0.03 mL/kg/cm H2O, in oxygen-exposed lungs when compared to the lungs exposed to air, 1.26±0.04, following a low pH aspirate (p<0.05). An increase in protein leakage into the lung tissue was noted in oxygen-exposed animals, PI=1.33±0.10, vs air-exposed rats, 0.89±0.07 (p<0.05). The hyperoxia-induced increase in lung injury was prevented by 30 mg/kg or higher deferoxamine treatment, 0.78±0.05 (p<0.05). Exposure of animals to 98% O2 for 2 h was sufficient to produce the same increase in microvascular protein leakage as 5-h exposure to O2 following low pH aspirate.
Conclusion: Hyperoxia increases acid aspiration-induced inflammatory microvascular lung injury. This appears to be mediated by production of reactive species of O2.
Key Words: acid aspiration • ARDS • deferoxamine • hyperoxia • neutrophil • oxygen radicals
Submitted on November 6, 1996
Accepted on April 3, 1997
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