| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Guest Access | Sign In via User Name/Password
|
|
|
|||||||
Guest Access | Sign In via User Name/Password |
|||||||||
1 From the Asthma and Allergic Diseases Center; and the Department of Medicine, University of Virginia School of Medicine, Charlottesville
2 From the Department of Student Health, University of Virginia School of Medicine, Charlottesville
3 From the Division of Epidemiology and Virology; and the Departments of Medicine and Pathology, University of Virginia School of Medicine, Charlottesville
Background: Exhaled oral nitric oxide (NO), a reported marker of inflammation in the respiratory tract, can be elevated by "upper respiratory tract infections." However, the responsible viruses and the time course of this rise in NO are not clear.
Objective: To determine the expired nasal and oral NO levels during experimentally induced influenza A infection in 14 healthy volunteers without a history of asthma, rhinitis, or sinusitis.
Methods: After being housed in individual rooms, susceptible volunteers were inoculated with 106 50% tissue culture infective dose of influenza A/Texas/36/91/(H1N1) virus on a single occasion by intranasal drops. Volunteers remained in the isolation unit for 8 days and returned for follow-up 21 days after inoculation. Symptom scores and nasal washing for viral culture were obtained daily. NO samples from the mouth and nose were obtained on days 0 through 4, 8, and 21 by having the patient perform a slow vital capacity maneuver through a plastic tube into a Mylar balloon.
Results: All patients had influenza virus cultured from nasal washings (12 of 14 on day 1, 14 of 14 by day 5). Patient symptom scores peaked on day 3 (mean±SE; 15.4±3.2) and returned to baseline by day 8. Preinfection exhaled nasal NO (right, 28.4±3.7 parts per billion [ppb]; left, 27.7±4.6 ppb) was significantly higher than oral NO (5.8 ppb; p<0.001). Exhaled oral NO was significantly elevated on day 8 postinoculation (12.9±0.8 ppb; p<0.01 Bonferroni) and returned to baseline at follow-up. Nasal NO levels showed a slight decrease on days 2 to 4 but returned to baseline by day 8.
Conclusion: Experimental influenza virus infection can increase oral but not nasal exhaled NO levels. The timing of exhaled NO changes suggests that NO does not contribute to illness manifestations directly.
Key Words: influenza A • nitric oxide • upper respiratory tract infection
Submitted on August 29, 1997
Accepted on February 6, 1998
This article has been cited by other articles:
|
|
 |
|
  A. Bhowmik, T. A. R. Seemungal, G. C. Donaldson, and J. A. Wedzicha Effects of exacerbations and seasonality on exhaled nitric oxide in COPD Eur. Respir. J., December 1, 2005; 26(6): 1009 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-M. Vints, E. Oostveen, G. Eeckhaut, M. Smolders, and W. A. De Backer Time-Dependent Effect of Nitrate-Rich Meals on Exhaled Nitric Oxide in Healthy Subjects Chest, October 1, 2005; 128(4): 2465 - 2470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Akerstrom, M. Mousavi-Jazi, J. Klingstrom, M. Leijon, A. Lundkvist, and A. Mirazimi Nitric Oxide Inhibits the Replication Cycle of Severe Acute Respiratory Syndrome Coronavirus J. Virol., February 1, 2005; 79(3): 1966 - 1969. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Message and S. L. Johnston Host defense function of the airway epithelium in health and disease: clinical background J. Leukoc. Biol., January 1, 2004; 75(1): 5 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. KHARITONOV and P. J. BARNES Exhaled Markers of Pulmonary Disease Am. J. Respir. Crit. Care Med., June 1, 2001; 163(7): 1693 - 1722. [Full Text] [PDF]
|
|
|
|
|
|
J. C. de JONGSTE and K. ALVING Gas Analysis Am. J. Respir. Crit. Care Med., August 1, 2000; 162(2): S23 - 27. [Full Text] [PDF]
|
|
|
|
|
|
H. DUPLAIN, C. SARTORI, M. LEPORI, M. EGLI, Y. ALLEMANN, P. NICOD, and U. SCHERRER Exhaled Nitric Oxide in High-Altitude Pulmonary Edema . Role in the Regulation of Pulmonary Vascular Tone and Evidence for a Role against Inflammation Am. J. Respir. Crit. Care Med., July 1, 2000; 162(1): 221 - 224. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Fichtlscherer and A. Mülsch MR Imaging of Nitrosyl-Iron Complexes: Experimental Study in Rats Radiology, July 1, 2000; 216(1): 225 - 231. [Abstract] [Full Text]
|
|
|
|
|
|
G. F. Rimmelzwaan, M. M. J. W. Baars, P. de Lijster, R. A. M. Fouchier, and A. D. M. E. Osterhaus Inhibition of Influenza Virus Replication by Nitric Oxide J. Virol., October 1, 1999; 73(10): 8880 - 8883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J O N Lundberg and E Weitzberg Nasal nitric oxide in man Thorax, October 1, 1999; 54(10): 947 - 952. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
