Chest ACCP Education Calendar
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Guest Access | Sign In via User Name/Password
This Article
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Article Archive
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arikan, S.
Right arrow Articles by Ustaçelebi, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arikan, S.
Right arrow Articles by Ustaçelebi, S.
(Chest. 1998;114:1253-1257.)
© 1998 American College of Chest Physicians

Anti-Kp 90 IgA Antibodies in the Diagnosis of Active Tuberculosis

Sevtap Arikan MD1; Serdar Tuncer MD1; Dürdal Us PhD1; Serhat Ünal MD1; and Scedilemsettin Ustaçelebi PhD1

1 From the Hacettepe University School of Medicine, Ankara, Turkey

Dürdal Us, PhD, Hacettepe University School of Medicine, Department of Clinical Microbiology and Microbiology, 06100 Ankara, Turkey

Background: Accurate diagnosis of active tuberculosis (TB) has been difficult historically, yet a great demand persists for a rapid and reliable diagnostic method. Detection of Mycobacterium tuberculosis anti-Kp 90 IgA antibodies is one of the more novel techniques.

Study objectives: To evaluate the diagnostic value of a recently developed enzyme-linked immunosorbent assay (ELISA) test, which detects IgA antibodies against M tuberculosis Kp 90 antigen, and to compare the results with conventional diagnosis and the polymerase chain reaction (PCR) method.

Participants: Serum, ethylenediaminetetraacetic acid (EDTA)-blood, and body fluid samples were obtained from 51 patients with active TB and 71 control subjects. The clinical diagnosis of TB was supported by a positive culture (n = 6), detection of acid-fast bacilli on smear (n = 35), or both (n = 10).

Measurements and results: IgA antibodies were detected in sera and/or body fluid samples from 82% of patients with TB and 10% of controls. M tuberculosis DNA was detected in body fluid sample of 96% and blood sample of 49% of patients with TB by PCR. None of the blood and 5.6% of the body fluid specimens from controls were PCR-positive.

Conclusions: Anti-Kp 90 IgA antibodies were detected using ELISA in 78% of serum and 69% of body fluids from patients with TB, therefore, this test is promising for the diagnosis of active TB and appears to be more reliable, particularly for body fluid samples.

Key Words: antigen Kp 90 • enzyme-linked immunosorbent assay • IgA • polymerase chain reaction • tuberculosis

Submitted on September 3, 1997
Accepted on May 5, 1998




This article has been cited by other articles:


Home page
 CVIHome page
R. P. Tiwari, D. Tiwari, S. K. Garg, R. Chandra, and P. S. Bisen
Glycolipids of Mycobacterium tuberculosis Strain H37Rv Are Potential Serological Markers for Diagnosis of Active Tuberculosis
Clin. Vaccine Immunol., March 1, 2005; 12(3): 465 - 473.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
M. B. Conde, P. Suffys, J. R. Lapa e Silva, A. L. Kritski, and S. E. Dorman
Immunoglobulin A (IgA) and IgG Immune Responses against P-90 Antigen for Diagnosis of Pulmonary Tuberculosis and Screening for Mycobacterium tuberculosis Infection
Clin. Vaccine Immunol., January 1, 2004; 11(1): 94 - 97.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
E. Julian, L. Matas, A. Perez, J. Alcaide, M.-A. Laneelle, and M. Luquin
Serodiagnosis of Tuberculosis: Comparison of Immunoglobulin A (IgA) Response to Sulfolipid I with IgG and IgM Responses to 2,3-Diacyltrehalose, 2,3,6-Triacyltrehalose, and Cord Factor Antigens
J. Clin. Microbiol., October 1, 2002; 40(10): 3782 - 3788.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
E. D. CHAN, R. REVES, J. T. BELISLE, P. J. BRENNAN, and W. E. HAHN
Diagnosis of Tuberculosis by a Visually Detectable Immunoassay for Lipoarabinomannan
Am. J. Respir. Crit. Care Med., May 1, 2000; 161(5): 1713 - 1719.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American College of Chest Physicians.