Chest Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Guest Access | Sign In via User Name/Password
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Article Archive
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maeda, H.
Right arrow Articles by Ueda, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maeda, H.
Right arrow Articles by Ueda, R.
(Chest. 2000;118:923-927.)
© 2000 American College of Chest Physicians

Human Herpesvirus 8 Is Not Associated With Sarcoidosis in Japanese Patients*

Hiroyoshi Maeda, MD; Takashi Niimi, MD; Shigeki Sato, MD; Haruhiko Kawaguchi, MD; Yoshiki Sugiura, MD; Shigeo Mori, MD and Ryuzo Ueda, MD

* From the Second Department of Internal Medicine (Drs. Maeda, Niimi, Sato, Kawaguchi, Sugiura, and Ueda), Nagoya City University Medical School, Nagoya, Japan; and the Department of Pathology (Dr. Mori), Institute of Medical Science, University of Tokyo, Tokyo, Japan.

Correspondence to: Dr. Hiroyoshi Maeda, Second Department of Internal Medicine, Nagoya City University Medical School, 1 Kawasumi, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.

Study objectives: The etiology of sarcoidosis remains unknown, but recently it was reported that human herpesvirus 8 (HHV-8) may be detected in sarcoid tissue in a high proportion of patients. This study was performed to determine whether HHV-8 is implicated in sarcoidosis in Japan.

Materials and methods: Peripheral blood mononuclear cells (PBMCs) were obtained from 100 patients with sarcoidosis and 100 healthy donors living in central Japan. Additionally, 19 samples of sarcoid tissue, 10 of tuberculous tissue, and 10 of lung cancers were examined. DNA was extracted from PBMC or tissue samples, and a hemi-nested polymerase chain reaction assay was performed for HHV-8 detection.

Results: In the PBMC study, the detection rates for HHV-8 in patients with sarcoidosis and in normal donors were 2% and 1%, respectively, the difference not being significant (p > 0.99). In the tissue study, HHV-8 was detected in 10.5% of sarcoid and in 15% of nonsarcoid tissues, again not a significant difference (p > 0.99).

Conclusions: From these results, we conclude that HHV-8 is not implicated in sarcoidosis in Japanese patients.

Key Words: human herpesvirus 8 • polymerase chain reaction • sarcoidosis




This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
Y. Eishi, M. Suga, I. Ishige, D. Kobayashi, T. Yamada, T. Takemura, T. Takizawa, M. Koike, S. Kudoh, U. Costabel, et al.
Quantitative Analysis of Mycobacterial and Propionibacterial DNA in Lymph Nodes of Japanese and European Patients with Sarcoidosis
J. Clin. Microbiol., January 1, 2002; 40(1): 198 - 204.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American College of Chest Physicians.