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(Chest. 2003;123:345S-348S.)
© 2003 American College of Chest Physicians

Isolation and Characterization of Hemopoietic Cells From Lungs of Allergic Mice*

Maria Ignez C. Gaspar Elsas, MD, PhD; Elisabeth S. Maximiano, PhD; Danielle Joseph; Adriana Bonomo, MD, PhD; Bernardo Boris Vargaftig, MD, PhD and Pedro Xavier Elsas, MD, PhD

* From the Departamento Pediatria (Dr. Elsas), Institute Fernandes Figueira, FIOCRUZ, Rio de Janeiro, Brazil; Department of Immunology (Drs. Maximiano, Bonomo, and Elsas), Institute Microbiologia Professor Paulo de Góes, UFRJ, Rio de Janeiro, Brazil; and Unité de Pharmacologie Cellulaire (Ms. Joseph and Dr. Vargaftig), Unité Associée Institut National de la Santé et de la Recherche Médicale-Institut Pasteur U485, Paris, France.

Correspondence to: P. Xavier Elsas, MD, PhD, Associate Professor, Department of Immunology, Instituto de Microbiologia Prof. Paulo de Góes, Universidad Federale do Rio de Janeiro, CCS, Bloco I, 2° andar, sala 066, 21941–590, Rio de Janeiro, Brazil; e-mail: pxelsas{at}yahoo.com.br

We developed a procedure for the isolation of hemopoietic cells from murine lung. Ovalbumin sensitization and challenge increased the numbers of functionally intact hemopoietic progenitors recovered from digested lung fragments by 80-fold to 120-fold, relative to naive controls. Eosinophil precursors, which are absent in the naive mouse lung, accumulated in the lungs of sensitized/challenged mice. Progenitors in allergic BALB/c mice were recoverable from lung parenchyma, not blood or airways, and were exclusively CD34+. Precursors isolated from allergic lung, unlike those from bone marrow, were inhibited by dexamethasone and were stimulated by prostaglandin D2. This directly demonstrates that sensitized/challenged lungs accumulate hemopoietic progenitors and precursors, distinct from those in bone marrow.






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