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* From the Department of Surgery (Drs. Hoang, D’Cunha, and Maddaus, and Ms. Casmey), Division of Cardiovascular and Thoracic Surgery, and the Thoracic Oncology Laboratory (Drs. M. Kratzke, Frizelle, and R. Kratzke), Division of Hematology, Oncology, and Transplant, Department of Medicine, University of Minnesota Medical School, Minneapolis, MN.
Correspondence to: Robert A. Kratzke, MD, Division of Hematology, Oncology, and Transplant, 111E MVAMC, 1 Veterans Dr, Minneapolis, MN 55417; e-mail: kratz003{at}tc.umn.edu
Study objective: We investigated the gene expression profiles of malignant pleural mesothelioma (MPM) specimens to identify novel genes that are potentially involved in the oncogenic transformation of human pleural cells.
Design: Complementary DNA (cDNA) microarray transcriptional profiling studies of 10 MPM cell lines and 4 MPM primary tumor specimens were performed using hierarchic clustering. To confirm microarray data, we used real-time polymerase chain reaction and immunoblotting.
Results: Cluster analysis differentiated among epithelial (E), sarcomatoid, and biphasic MPM variants. Expression profiling identified common overexpressed or underexpressed genes in MPM. Notably, matriptase messenger RNA was found to be overexpressed by 826-fold in E MPM, with protein expression subsequently confirmed by immunoblot analysis. This recently characterized trypsin-like serine protease has been implicated in tumor invasion and metastasis of E-derived cancers, but has not been described until now in MPM. We also identified other novel genes, such as insulin-like growth factor binding protein 5 and a cDNA clone similar to proteolipid MAL2.
Conclusions: Thus, further large-scale profiling of MPM may elucidate previously unrecognized molecular mechanisms by identifying novel genes that are involved in malignant transformation. Our study has now found matriptase to be one of these mesothelioma-associated genes, with potential pathogenic and therapeutic significance.
Key Words: expression profiling • matriptase • mesothelioma • microarray • real-time polymerase chain reaction
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