HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:
Guest Access | Sign In via User Name/Password

This Article Full Text Free Full Text (PDF) Free Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to My Personal Article Archive Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Basoglu, O. K. Articles by Kharitonov, S. A. Search for Related Content PubMed PubMed Citation Articles by Basoglu, O. K. Articles by Kharitonov, S. A.

Effects of Aerosolized Adenosine 5'-Triphosphate vs Adenosine 5'-Monophosphate on Dyspnea and Airway Caliber in Healthy Nonsmokers and Patients With Asthma^*

^* From the Section of Airway Diseases (Drs. Basoglu, Essilfie-Quaye, Brindicci, Barnes, and Kharitonov), National Heart and Lung Institute, Faculty of Medicine, Imperial College, Dovehouse St, London, UK; and Duska Scientific Co. (Dr. Pelleg), Bala Cynwyd, PA.

Correspondence to: Sergei A. Kharitonov, MD, PhD, Section of Airway Disease, National Heart & Lung Institute, Dovehouse St, London SW3 6LY, UK; e-mail: s.kharitonov{at}imperial.ac.uk

Study objectives: Extracellular adenosine 5'-triphosphate (ATP) causes neurogenic bronchoconstriction, inflammation, and coughs, and may play a mechanistic role in obstructive airway diseases. The aims of this study were to determine the effects of inhaled ATP on airway function, and to compare these effects with those of adenosine 5'-monophosphate (AMP).

Design: Prospective, randomized, double-blind study.

Setting: Clinical research laboratory of a postgraduate teaching hospital.

Methods: The effects of inhaled equimolar doses of ATP and AMP on airway caliber, perception of dyspnea quantified by the Borg score, and other symptoms were determined in 10 nonsmokers (age 41 ± 3 years) and 10 patients with asthma (age 39 ± 3 years) [± SEM].

Results: None of the healthy nonsmokers responded to ATP or AMP. All the patients with asthma responded to ATP, and 90% responded to AMP. The geometric mean of the provocative dose causing a 20% fall in FEV₁ (PD₂₀) of ATP was 48.7 µmol/mL and that of PD₂₀ AMP was 113.5 µmol/mL in responsive asthmatics (p < 0.05). In asthmatic patients, the percentage change in FEV₁ caused by ATP was greater than that caused by AMP (FEV₁ ATP = 29% vs FEV₁ AMP = 22%, p < 0.05). Borg score increased significantly in asthmatics after ATP (from 0.1 to 3.3, p < 0.01) and after AMP (from 0.2 to 2.5, p < 0.01). This increase was also greater after ATP than AMP in asthma (Borg ATP = 3.2 vs Borg AMP = 2.3, p < 0.05). ATP induced cough in 16 subjects (80%), while AMP induced cough in 8 subjects (40%) [p < 0.05]; in addition, more subjects had throat irritation after inhalation of ATP than AMP (p < 0.05).

Conclusions: ATP is a more potent bronchoconstrictor and has greater effects on dyspnea and other symptoms than AMP in asthmatic patients. Therefore, ATP could potentially be used as a bronchoprovocator in the clinical setting.

Key Words: adenosine 5'-monophosphate • adenosine 5'-triphosphate • airway caliber • asthma • Borg score • dyspnea • P2 receptors

This article has been cited by other articles:

				B. Chuaychoo, M.-G. Lee, M. Kollarik, R. Pullmann Jr, and B. J. Undem Evidence for both adenosine A1 and A2A receptors activating single vagal sensory C-fibres in guinea pig lungs J. Physiol., September 1, 2006; 575(2): 481 - 490. [Abstract] [Full Text] [PDF]