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(Chest. 2006;129:431-437.)
© 2006 American College of Chest Physicians

Oxidative Changes of Bronchoalveolar Proteins in Cystic Fibrosis*

Vitaliy Starosta, MD; Ernst Rietschel, MD; Karl Paul, MD; Ulrich Baumann, MD and Matthias Griese, MD

* From the Lung Research Group (Drs. Starosta and Griese), Children's Hospital of Ludwig Maximilians University, Munich; Department of Pediatric Pneumology and Allergology (Dr. Rietschel), Childrens’ Hospital University of Cologne, Cologne; Department of Pediatric Pneumology and Immunology (Dr. Paul), Charité, Humboldt-University, Berlin; and Department of Pediatric Pulmonology and Neonatology (Dr. Baumann), Hannover Medical School, Hannover, Germany.

Correspondence to: Matthias Griese, Childrens' Hospital, Ludwig-Maximilians-University, Lindwurmstr 4, D-80337 Munich, Germany; e-mail: matthias.griese{at}med.uni-muenchen.de

Abstract

Chronic bacterial infection and severe, polymorphonuclear neutrophil-dominated endobronchial inflammation are characteristic hallmarks of cystic fibrosis (CF) lung disease. The free radicals generated can be deleterious for structure and function of many proteins. The goal of this study was to investigate the degree of oxidation of pulmonary epithelial lining fluid proteins. BAL fluid (BALF) from 55 children with CF and from 11 patients in a control group were investigated by dot-blot assay for content and by two-dimensional electrophoresis and Western blotting for the pattern of distribution of oxidized proteins. The highest level of oxidative stress, as assessed by the level of protein carbonyls, was found in patients with FEV1 < 80% of predicted or with highly elevated neutrophil counts. Compared to control subjects without lung disease, CF patients with normal lung function and CF patients with a normal neutrophil count in their BALF had significantly higher protein carbonyl levels. The extent of protein oxidation was directly related to the neutrophil granulocyte count and inversely to lung function. Our data support the hypothesis that oxidative damage of pulmonary proteins during chronic and excessive neutrophilic endobronchial inflammation may contribute to the decline of lung function in CF patients.

Key Words: cystic fibrosis • lung disease • protein carbonyls






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