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doi:10.1378/chest.06-2031
(Chest. 2007; 131:1149-1156)
© 2007 American College of Chest Physicians
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Niflumic Acid and AG-1478 Reduce Cigarette Smoke-Induced Mucin Synthesis*

The Role of hCLCA1

Ahmed E. Hegab, PhD; Tohru Sakamoto, MD; Akihiro Nomura, MD; Yukio Ishii, MD; Yuko Morishima, MD; Takashi Iizuka, MD; Takumi Kiwamoto, MD; Yosuke Matsuno, MD; Shinsuke Homma, MD and Kiyohisa Sekizawa, MD

* From the Department of Pulmonary Medicine, Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.

Correspondence to: Tohru Sakamoto, MD, Department of Pulmonary Medicine, Institute of Clinical Medicine, University of Tsukuba, 1–1-1 Tennoudai, Tsukuba, Ibaraki 305-8575, Japan; e-mail: t-saka{at}md.tsukuba.ac.jp

Abstract

Background: Cigarette smoke induces bronchial mucus secretion. However, the mechanism of this induction is still unidentified. In this study, we investigated the role of the putative calcium-activated chloride channel 1 (CLCA1) and its blocker, niflumic acid, in cigarette smoke-induced mucin synthesis both in vivo and in vitro.

Methods and results: Sprague-Dawley rats were exposed to cigarette smoke for 4 weeks. The CLCA1, epidermal growth factor receptor (EGFR), and MUC5AC expressions were increased in the trachea and lung tissues. Goblet-cell hyperplasia with marked mucin staining was detected in the tracheal and bronchial epithelium. In the human bronchial epithelial cell line NCI-H292, cigarette smoke solution also induced mucin production as well as the RNA and protein expressions of CLCA1, EGFR, and MUC5AC. Both in vivo and in vitro, the induction of MUC5AC and mucin synthesis were inhibited by niflumic acid, and/or a selective EGFR tyrosine kinase inhibitor, AG-1478. Niflumic acid also blocked the epidermal growth factor-induced MUC5AC and mucin staining in the NCI-H292 cell line.

Conclusion: Both EGFR and niflumic acid-sensitive chloride channels (probably CLCA1) are dependently affecting the mucin production as a part of a single complex signaling pathway. CLCA1 may be a key signaling member that can be targeted with pharmacologic interventions to treat mucus hypersecretion.

Key Words: calcium-activated chloride channel • COPD • epidermal growth factor receptor • MUC5AC






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