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(Chest. 1966;50:630-632.)
© 1966 American College of Chest Physicians

The Effect of Anticontamination Agents in Media for the Isolation of Mycobacteria

Victor Lorian M.D.1 and Stephen Maddock M.D.1

1 Sanatorium Division, Boston City Hospital, Mattapan, and Boston University School of Medicine, Infectious Disease Section

A comparative study for mycobacterial growth, sensitivity tests, and contamination was done on collection strains of mycobacteria and for 1625 sputum specimens planted on 7H10 agar and the same medium to which colimycin, spiramycin, and amphotericin B were added. The collection strains showed equal growth and equal sensitivity results on 7H10 media with or

See Table in the PDF File

without the addition of the three mentioned anticontamination agents. The 7H10 agar containing colimycin, spiramycin, and amphotericin B compared to the 7H10 agar alone showed 10 per cent less contamination on the sputum cultures and 70 per cent less contamination for the experimental work where air contamination was tested.






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Copyright © 1966 by the American College of Chest Physicians.