Chest ACCP Career Connection
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Guest Access | Sign In via User Name/Password
This Article
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Article Archive
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miserocchi, G.
Right arrow Articles by De Luca, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miserocchi, G.
Right arrow Articles by De Luca, G.
(Chest. 1999;116:31S.)
© 1999 American College of Chest Physicians

Interstitial Pressure and Proteoglycan Degradation in Hydraulic- and Elastase-Induced Lung Edema*

G. Miserocchi, MD; A. Passi; R. Albertini; D. Negrini and G. De Luca

* From the Istituto di Fisiologia Umana, Università degli Studi, Milano, Italy, and the Dipartimento di Biochimica "A. Castellani," Università degli Studi, Pavia, Italy.

Correspondence to: G. Miserocchi, MD, Istituto di Fisiologia Umana, Universita di Milano, Via Mangiagalli 32, 20133 Milano, Italy.

In anesthetized rabbits, pulmonary interstitial pressure (Pip) was measured by micropuncture technique performed through a pleural window during the development of lung edema induced either by IV saline solution loading or elastase administration (7 IU). In control condition, Pip at heart level was -10 ± 2 (1 SD) cm H2O. In both edema models, Pip increased to about +5 cm H2O (phase of interstitial edema) and then dropped to about 0 cm H2O in severe edema, indicating a loss of the mechanical integrity of pulmonary tissue matrix.1 2 3 Pip correlated to structural and functional modifications of proteoglycans (PGs), the major interfibrillar components of extracellular matrix (ECM). In severe edema, PG ability to bind other ECM components was markedly reduced, and electrophoretic and gel-filtration analyses showed a PG breakdown, mainly affecting the protein moiety. Qualitative zymography performed on lung extracts showed two main gelatinolytic bands at about 92 kd and 72 kd, identified by specific antisera as progelatinase B and progelatinase A, respectively and, in addition, two minor bands corresponding to their proteolytically activated forms. The intensity of all bands and the relative proportions of the proteolytically activated forms increased markedly with edema development. The results suggest that the development of hydraulic- and elastase-induced pulmonary edema is associated with an increase of endogenous protease activities, which eventually leads to a loss of the native architecture of ECM.

References

  1. Miserocchi, G, Negrini, D, Del Fabbro, M, et al (1993) Pulmonary interstitial pressure in intact in situ lung: transition to interstitial edema. J Appl Physiol 74,1171-1177[Abstract/Free Full Text]
  2. Negrini, D, Passi, A, de Luca, G, et al (1996) Pulmonary interstitial pressure and proteoglycans during development of pulmonary edema. Am J Physiol 270,H2000-H2007[Abstract/Free Full Text]
  3. Passi, A, Negrini, D, Albertini, R, et al (1998) Involvement of lung interstitial proteoglycans in development of hydraulic- and elastase-induced edema. Am J Physiol 275,L631-L635[Abstract/Free Full Text]




This Article
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Article Archive
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miserocchi, G.
Right arrow Articles by De Luca, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miserocchi, G.
Right arrow Articles by De Luca, G.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS