Chest ACCP Education Calendar
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Guest Access | Sign In via User Name/Password
This Article
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Article Archive
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, A. Q.
Right arrow Articles by Loyd, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, A. Q.
Right arrow Articles by Loyd, J. E.
(Chest. 2002;121:25S.)
© 2002 American College of Chest Physicians

Allelic Imbalance Demonstrated by Microsatellite Analysis of Lung Samples From Patients With Familial Pulmonary Fibrosis*

Alan Q. Thomas, MD; Jennifer Carneal, BS; Cheryl Markin, BS; Kirk B. Lane, PhD; John A. Phillips, III, MD and James E. Loyd, MD

* From the Division of Allergy, Pulmonary, and Critical Care Medicine (Drs. Thomas, Lane, Loyd, and Ms. Markin), and Division of Medical Genetics (Ms. Carneal and Dr. Phillips), Vanderbilt University School of Medicine, Nashville, TN.

Correspondence to: Alan Q. Thomas, MD, Research Fellow, Center for Lung Research, Department of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University School of Medicine, 1211 22nd Ave, Nashville, TN 37232

Idiopathic pulmonary fibrosis (IPF) is a fatal, proliferative lung disease of unknown etiology. The relative risk of lung cancer in patients with IPF is seven times that of smoking control subjects, suggesting chronic DNA damage and/or repair dysfunction. Vassilakis et al1 demonstrated loss of heterozygosity (LOH) and microsatellite instability (MSI) in the DNA from sputum of patients with IPF. We hypothesized that LOH and MSI might be involved in the pathogenesis of familial IPF (FIPF) and could possibly identify candidate loci. Thus, we analyzed the incidence of LOH and MSI at six highly polymorphic microsatellite marker loci from 11 patients with FIPF and 4 patients with familial primary pulmonary hypertension. Microsatellite markers were analyzed on an ABI 310. Applied Biosystems, Foster City, CA). The allele ratio of each patient was calculated using the following previously described formula: (T1/T2)/(N1/N2), where N1 and N2 are areas under the allele peaks in blood, and T1 and T2 are areas under the allele peaks in lung tissue. Four of 11 patients with FIPF (36%) exhibited LOH by at least one marker, including D17S579, D9S59, and D8S133, all adjacent to putative tumor suppressor genes. No patients with familial primary pulmonary hypertension showed LOH, and MSI was not detected in either group. These findings suggest that allelic imbalance occurs in patients with FIPF patients, which may play a role in the pathogenesis of the disease and identify candidate loci for relevant genes.


   Footnotes
 
Abbreviations: FIPF = familial idiopathic pulmonary fibrosis; IPF = idiopathic pulmonary fibrosis; LOH = loss of heterozygosity; MSI = microsatellite instability

This work was supported by HL-07123 and by the Vanderbilt University Discovery Program.


   References
TOP
 References
 

  1. Vassilakis, DA, Sourvinos, G, Pantelidis, P, et al (2001) Extended genetic alterations in a patient with pulmonary sarcoidosis, a benign disease. Sarcoidosis Vasc Diffuse Lung Dis 18,307-310[Medline]



This article has been cited by other articles:


Home page
 ChestHome page
J. W. Huh, D. S. Kim, Y.-M. Oh, T. S. Shim, C. M. Lim, S. D. Lee, Y. Koh, W. S. Kim, W. D. Kim, and K. R. Kim
Is Metalloproteinase-7 Specific for Idiopathic Pulmonary Fibrosis?
Chest, May 1, 2008; 133(5): 1101 - 1106.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Article Archive
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, A. Q.
Right arrow Articles by Loyd, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, A. Q.
Right arrow Articles by Loyd, J. E.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS