HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:
Guest Access | Sign In via User Name/Password

This Article Full Text (PDF) Free Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to My Personal Article Archive Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Vassallo, R. Articles by Chen, L. Search for Related Content PubMed PubMed Citation Articles by Vassallo, R. Articles by Chen, L.

Selective Suppression of Dendritic Cell Functions by Cigarette Smoke Extract^*

^* From the Mayo Clinic, Rochester, MN.

Correspondence to: Robert Vassallo, MD, FCCP, Assistant Professor of Medicine, Division of Pulmonary and Critical Care Medicine, Mayo Clinic, Stabile Bldg 8–54, Rochester, MN 55905; e-mail: vassallo.robert{at}mayo.edu

Dendritic cells are potent antigen-presenting cells and critical regulators of T-cell responses. We postulated that an important mechanism by which smoking predisposes a person to cancer is by suppressing dendritic cell function, thus inhibiting the ability of dendritic cells to stimulate appropriate T-cell responses. To test this hypothesis, human monocyte-derived dendritic cells were incubated with freshly prepared cigarette smoke extract (CSE) [0 to 2%; mean (± SD) nicotine content in 1% CSE, 2.3 ± 0.8 µg/mL] for the final 18 to 24 h of culture. Dendritic cells were then washed to remove all residual CSE and were incubated with allogenic purified T cells in a mixed lymphocyte reaction for 72 h. T-cell proliferation was measured by the incorporation of tritiated thymidine and cytokine levels into the mixed lymphocyte reaction measured by enzyme-linked immunosorbent assay. The transient incubation of immature dendritic cells with CSE at doses that did not affect viability (as measured by the XTT assay, trypan blue exclusion, and annexin V/propidium iodide staining) resulted in significant suppression of dendritic cell-induced T-cell proliferation. This decrease in T-cell proliferation was accompanied by reduced interferon- production, but not in interleukin-4 or interleukin-10 secretion. Dendritic cell surface expression of major histocompatibility complex class II, major histocompatibility complex class I, CD-40, CD-80, CD-86, CD-83, and CD-1a was not altered by transient exposure (ie, for 24 h) to CSE at the same concentrations that diminished dendritic cell-induced T-cell proliferation. These data indicate that transient exposure to CSE causes the selective suppression of dendritic cell functions in the induction of T-cell proliferation and the production of interferon-.

	Footnotes

 
Abbreviation: CSE = cigarette smoke extract

This research was supported by an American Lung Association research grant, a Parker B. Francis fellowship (RV), and National Institutes of Health grants CA79915 and CA85721 (LC).

This Article Full Text (PDF) Free Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to My Personal Article Archive Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Vassallo, R. Articles by Chen, L. Search for Related Content PubMed PubMed Citation Articles by Vassallo, R. Articles by Chen, L.